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Basic Research Journal of Microbiology ISSN 2354-4082 Vol. 4(4), pp. 033-041 July 2017

Copyright© 2017 Basic Research Journals

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Full Length Research Paper
 

Use of chloramphenicol in the differential enumeration of greenish pigment producing Pseudomonas

 

Lekiah P. Peekate1* and Gideon O. Abu2

 

1Department of Microbiology, Faculty of Science, Rivers State University, P. M. B. 5080 Port Harcourt, Nigeria.

2Department of Microbiology, Faculty of Science, University of Port Harcourt, P. M. B. 5323, Port Harcourt, Nigeria.

 

*Corresponding author email: lekia.peekate@ust.edu.ng, gideon.abu@uniport.edu.ng

 

Received 29 June, 2017; Accepted 11 July, 2017; Published 16 July, 2017

 

Abstract

 

Cultures of Pseudomonas are often required for research, biotechnology, industrial application, and environmental application. Sourcing for species of Pseudomonas from environmental samples can be made easy by using a procedure that promotes production of greenish pigments in selected species of Pseudomonas. Differential enumeration of greenish pigment producing bacteria in river water, samples was carried out using separate nutrient agar plates containing 50µg.ml-1, and 100µg.ml-1 Chloramphenicol. Greenish pigment producing colonies were obtained on nutrient agar plates containing 50µg.ml-1 Chloramphenicol after 48 hours of incubation at ambient temperature. The proportion of the bacterial population producing greenish pigments on the plates ranged from 11.11% - 30.77%. Results generated from Gram staining and physicochemical/biochemical tests carried out on selected isolated colonies which produced green pigments revealed that the isolates are all P. fluorescens. Also, selected results generated for one of the isolate which was submitted to an internet based bacterial identification software showed that the isolate is 89% related to P. fluorescens. Biomolecular identification via 16S rRNA gene sequencing suggested that the isolate is P. fluorescens. The procedure used in this research provides a means of identifying environmental samples containing Pseudomonas and the subsequent isolation of the bacterium.

 

Keywords: Pseudomonas, P. fluorescens, Greenish pigment, Chloramphenicol, Differential enumeration

 

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Vol.4 No 4

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