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Basic Research Journal of Microbiology ISSN 2354-4082 Vol. 3(5), pp. 30-38 October 2016

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Full Length Research Paper

Identification of contaminating microorganisms during collection of umbilical cord blood for transplantation


Jaime Vargas-Arzola1, Sergio R. Aguilar-Ruiz2, Luis Alberto Hernández Osorio1, Honorio Torres-Aguilar1,3,*


1Chemical Sciences Faculty, Autonomous University “Benito Juárez”, Oaxaca City, Oaxaca, Mexico

*2Research Center of Medical and Biological Sciences, Medicine and Surgery Faculty, Autonomous University “Benito Juárez”, Oaxaca City, Oaxaca, Mexico

3Research Sub-direction, National Center of Blood Transfusion, Mexico City, Mexico.


*Corresponding author email: qbhonorio@hotmail.com


Received 26 October, 2016; Accepted 04 November, 2016; Published 13 October, 2016




Umbilical cord blood is a source of hematopoietic and mesenchymal stem cells for cellular therapy. Procedures to purify and to preserve these cells are performed under strictly controlled sterile conditions, but some units may become contaminated during collection. Ninety-six units detected as contaminated during an initial screening by Bact/Alert®3D60 (of 2,391 units collected from 2003 to 2012) were subjected to microbiologic evaluation by biochemical tests or Vitek-2 system to elucidate the probable origin of contamination. The most frequently isolated bacteria were from normal gastrointestinal flora such as Escherichia coli (24.6%), Enterococcus sp [faecium (19.7%), faecalis (4.9%) and gallinarum (4.9%)]; Citrobacter freundii (6.5%); Klebsiella pneumonia (4.9%); Proteus mirabilis (3.3) and Enterobacter cloacae (1.6%). Candida albicans (19.7%) was the one yeast found. Normal constituent of the human skin and environmental bacteria were isolated: Klebsiella pneumoniae (4.9%) and Acinetobacter calcoaceticus 1.6%). Pantoea agglomerans (3.3%), Cronobacter sakazakii (1.6%), Leclercia adecarboxylata (1.6%) and Staphylococcus lentus (1.6%). The most probable contaminations sources were produced by cross contamination with perineal/enteric, vaginal or environmental microorganisms after delivering. The appointment of a specific trained person dedicated exclusively to blood collection and paying special attention on umbilical cord sanitization before puncturing might significantly reduce contamination.


Keywords: Umbilical cord blood, stem cells, transplantation, cross contamination.


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