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BASIC RESEARCH JOURNALS OF AGRICULTURAL SCIENCE AND REVIEW ISSN 2315-6880
 

Basic Research Journal of Agricultural Science and Review ISSN 2315-6880 Vol. 8(1), pp. 015-021 July 2020 Copyright© 2020 Basic Research Journals

International Scientific Indexing (Impact Factor (0.401))

Thompson Reuters ResearchersID A-9294-2018

 

Full Length Research Paper
 

Effect of various physical factors, chemical disinfectants and herbal extracts on the infectivity of Peste des Petits ruminants virus

 

Kinza Khan¹, Muti-ur-Rehman Khan² and *Asad Ali Khan³

 

¹Lecturer  Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan.

²Department of Pathology, University of Veterinary and Animal Sciences, Lahore.

³Professor Department of Geography, The Islamia University of Bahawalpur

 

Corresponding author email: asadkhaniub@yahoo.com

 

Received 17 July, 2020; Accepted 24 July 2020; Published 31 July, 2020

 

Abstract

 

Peste des petits ruminants (PPR) is a contagious viral disease of small ruminants found in various parts of the world including Pakistan. Present study is focused on to evaluate the effect of various physical factors, chemical disinfectants and herbal extracts on the infectivity of PPR virus (PPRV). The virus as a cell culture harvest was exposed to the physical or chemical agent and residual viral infectivity was measured at regular intervals. Complete inactivation of the virus was achieved at 56°C after 10 minutes of exposure while at 37, 25, and 4°C a drop of 1.00, 1.30, and  2.85 log10 of the virus infectivity was recorded after 2 hours, 24 hours, and 10 days, respectively. After exposing the virus to pH values of 6.00, 7.40 and 8.00 for 2 hours, a drop of 2.00, 1.30, and 2.08 log10 of the virus infectivity was recorded. Similarly the virus was completely inactivated when exposed to ultraviolet radiations for 12 hours. With a starting virus concentration of <5.00 tissue culture infective dose50 (TCID50) /ml, a minimum of 0.25% virusnip and 0.001% formaldehyde completely inactivated the virus within 5 minutes and 2 hours respectively while negligible viral infectivity was still detectable after an exposure of 60 minutes to 0.50% pyodine solution. It revealed that herbal extracts were relatively less effective in reducing the virus infectivity. Exposure to mentofin (0.5%), calvangi oil (0.5%) and Opuntia dellinii (10%) for 24 hours resulted into the reduction of 3.89, 1.63, and 1.61 log10 virus infectivity.

 

Keywords: Contagious viral disease, Chemical disinfectants, Herbal extract, pH, PPRV, Temperature, UV light.

 

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Basic Research Journal of Business Management and Accounts.

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Basic Research Journal of Medicine and Clinical Sciences.

Basic Research Journal of Social and Political Sciences.

J. Agric. Sci. Rev

Vol.8 No 2

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